Calprotectin Semi-quantitative Rapid Test

 

Calprotectin, first described in 1980^[1], is aprotein found in the cytosol of neutrophils and macrophages composed of two subunits S100A8 and S100A9. It is released extracellularly in times of cell stress or damage and can be detected within feces and thus can be used as a sensitive marker of intestinal inflammation. It is stable in feces for up to seven days at room temperature and has a homogenous distribution in feces^[2]
[3][4] and ulcerative colitis, are chronic relapsing, remitting disorders. Diagnosis along with assessment of disease activity and prognosis present challenges to managing clinicians. Fecal biomarkers, such as fecal calprotectin, are a non-invasive method which can be used to aid the sedecisions. Fecal protectin has been shown to be useful in the diagnosis of IBD, correlates with bowel mucosal disease activity and can help to predict
^[5].

 

Allow test cassette, specimen, and/or controls to equilibrate to room temperature (15-30 degree ) prior to testing.
1. To collect fecal specimens:
Collect sufficient quantity of feces (1-2 mL or 1-2 g) in a clean, dry specimen collection container to obtain enough virus particles. Best results will be obtained if the assay is performed within 6 hours after collection. Specimen collected may be stored for 3 days at 2-8 degree if not tested within 6 hours. For long term storage, specimens should be kept below -20 degree .
2. To process fecal specimens:
Unscrew the cap of the specimen collection tube, then randomly stab the specimen collection applicator into the fecal specimen in at least 3 different sites to collect approximately 10~50 mg of feces (equivalent to 1/4 of a pea). Do not scoop the fecal specimen.
3. Bring the pouch to room temperature before opening it. Remove the test cassette from the sealed pouch and use it as soon as possible. Best results will be obtained if the assay is performed within one hour.
4. Place the cassette on a clean and level surface Hold the specimen collection tube upright and unscrew the tip of the specimen collection tube. Invert the specimen collection tube and transfer 2 full drops of the extracted specimen (approximately 80uL) to the specimen well (S) of the test cassette, then start the timer. Avoid trapping air bubbles in

5. Wait for the colored line(s) to appear. The test result should be read at 10 minutes. Do not interpret the result after 15 minutes.